Mitochondrial CoQ deficiency is a common driver of mitochondrial oxidants and insulin resistance

نویسندگان

  • Daniel J Fazakerley
  • Rima Chaudhuri
  • Pengyi Yang
  • Ghassan J Maghzal
  • Kristen C Thomas
  • James R Krycer
  • Sean J Humphrey
  • Benjamin L Parker
  • Kelsey H Fisher-Wellman
  • Christopher C Meoli
  • Nolan J Hoffman
  • Ciana Diskin
  • James G Burchfield
  • Mark J Cowley
  • Warren Kaplan
  • Zora Modrusan
  • Ganesh Kolumam
  • Jean Yh Yang
  • Daniel L Chen
  • Dorit Samocha-Bonet
  • Jerry R Greenfield
  • Kyle L Hoehn
  • Roland Stocker
  • David E James
چکیده

Insulin resistance in muscle, adipocytes and liver is a gateway to a number of metabolic diseases. Here, we show a selective deficiency in mitochondrial coenzyme Q (CoQ) in insulin-resistant adipose and muscle tissue. This defect was observed in a range of in vitro insulin resistance models and adipose tissue from insulin-resistant humans and was concomitant with lower expression of mevalonate/CoQ biosynthesis pathway proteins in most models. Pharmacologic or genetic manipulations that decreased mitochondrial CoQ triggered mitochondrial oxidants and insulin resistance while CoQ supplementation in either insulin-resistant cell models or mice restored normal insulin sensitivity. Specifically, lowering of mitochondrial CoQ caused insulin resistance in adipocytes as a result of increased superoxide/hydrogen peroxide production via complex II. These data suggest that mitochondrial CoQ is a proximal driver of mitochondrial oxidants and insulin resistance, and that mechanisms that restore mitochondrial CoQ may be effective therapeutic targets for treating insulin resistance.

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عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2018